Objective：To analyze differences in expressions of CPAF, IFN-γ and IL-10 by establishing mouse genital tract infection with different CT genotypes. Methods： Seventy female Balb/c mice were randomly divided into E group, F group, J group, K group, H group, blank control group and experimental control group. Chlamydia trachomatis genotypes E, F, J, K and H (30 μL)  were inoculated into vagina respectively.The blank control group was not treated,while the experimental control group was inoculated into vagina with 30 μL SPG. Expression of CPAF, IFN-γ and IL-10 in the uterus and fallopian tube was detected by ELISA on day 35 postinoculation. Results: On day 35 postinoculation, the concentration of CPAF in homogenate of uterus and fallopian tube in group H was significantly higher than that in group E, F and J (all P<0.05). The concentration of CPAF in group K was significantly higher than that in group F (P<0.05). The concentration of IFN-γ and IL-10 in uterus and fallopian tube homogenate in group H and K were significantly higher than that in group E, F and J (all P<0.05).Conclusion: The expression of CPAF, IFN-γ and IL-10 in the upper genital tract of mice infected with different types of CT show differences significantly, indicating that different genotypes of CT may cause different clinical outcomes.

Objective：To explore the value of dermoscopy in the diagnosis of seborrheic keratosis, actinic keratosis and basal cell carcinoma.Methods：Patients which clinically diagnosed as seborrheic keratosis  (108 cases), actinic keratosis  (46 cases) and basal cell carcinoma (71 cases) were enrolled, and their clinical data, clinical pictures, dermoscopic diagnosis and pathological findings were collected. The sensitivity, specificity and consistency  of  dermoscopy in the diagnosis of these three diseases  were analyzed. Results: The consistency, sensitivity, specificity, misdiagnosis rate,  missed diagnosis rate, Youden index and Kappa index were  reported as followings: ①for seborrheic keratosis, were 93.52%、93.52%, 94.67%、93.33%,90.91%、93.94%,9.09%、6.06%，5.33%、6.67%,85.58%、87.27%,0.8484、0.8511, respectively. ②for actinic keratosis, were 89.13%、89.13%,88.89%、85.19%,89.47%、94.74%,10.53%、5.26%,11.11%、14.81%, 78.36%、79.93%,0.7776、0.7809, respectively. ③for basal cell carcinoma, were 91.55%、91.55%,89.74%、92.31%,93.75%、90.63%,6.25%、9.37%,10.26%、7.69%,83.49%、74.84%, 0.8304、0.8293, respectively. Conclusion: The application of dermoscopy in the diagnosis of seborrheic keratosis, actinic keratosis and basal cell carcinoma has a high diagnostic value, which is worthy of clinical application.

Objective：To investigate the expression of IL-33 in serum and skin lesions of patients with progressive psoriasis vulgaris (PsV) and its clinical significance. Methods： A total of 126 patients with PsV and 45 healthy individuals were enrolled from January 2018 to January 2019 which defined as PsV group and control group, respectively. The differences of IL-33 expression in serum between the two groups were analyzed and compared,and the differences of IL-33 expression in skin tissue between patients with PsV and lipoma patients were also compared. The serum IL-33 expression levels with PASI scores correlation, differences between clinical characteristics and influencing factors in patients with PsV were analyzed by Pearson correlation analysis, single factor analysis and logistic regression analysis, respectively. Receiver Operating Characteristic (ROC) curve was used to investigate the clinical efficacy of serum IL-33 levels in differentiating PsV from control group, mild from moderate to severe PsV. Results: Compared with the control group, the expression of IL-33 in the serum of the patients with PsV was significantly higher (t=17.51, P<0.01), and the expression of IL-33 in the skin lesions of the patients with moderate to severe PsV (16.56±4.18) was significantly higher than that of the patients with mild PsV (9.28±1.04) and the lipoma patients (5.64±1.45) (t=7.56、11.04, respectively,both P<0.05). The expression of IL-33 in the skin lesions of the patients with mild PsV were significantly higher than that of the lipoma patients (t=9.12, P<0.05). Pearson correlation analysis showed that serum IL-33 was positively correlated with PASI scores (r=0.85, P<0.01). The serum IL-33 expression levels was different significantly between different BMI, course of disease and severity of skin lesions of patients with PsV. Duration (OR=3.449), PASI (OR=10.444) were positively correlated to serum IL-33 levels in patients with PsV. ROC curve showed that serum IL-33 levels in differentiating progressive PsV from control group, mild from moderate to severe progressive PsV were significantly. The AUC was 0945 and 0870, respectively. Conclusions: The expression of IL-33 in serum and lesions of patients with PsV were significantly increased, and IL-33 may be involved in the pathogenesis of psoriasis.

Objective：To detect the expression of C/EBPβ protein in the lesions of patients with psoriasis vulgaris by  western blot. Methods： C/EBPβ protein expression levels of tissues from 15 psoriatic lesions and 10 healthy controls were determined by the means of whole protein extraction and Western blot, and the correlation between expression of C/EBPβ and PASI scores was analyzed. Results: C/EBPβ protein expression  （0.84±0.41）  was significantly decreased in psoriasis compared to the normal control (t=2.12,P＜0.05). Pearson correlation analysis showed that C/EBPβ protein expression level was negatively correlated with PASI score(r=-0.66，P＜0.05).Conclusion: C/EBPβ protein was down expression in psoriatic lesions, which established foundation to further reveal the mechanism of C/EBPβ protein involved in the pathogenesis of psoriasis vulgaris.

Objective：To compare  the   application value of the fluorescence staining method with  KOH wet method in superficial fungal infections of the skin.   Methods： One hundred and ninety two outpatient suspected patients  of superficial fungal skin infection were collected. Specimens were divided into two parts, and detected by   fluorescent staining and KOH wet method  at the same time, then  the test results were compared. Result: With fluorescent staining method: the positive rate was 73.4% (141/192). In the patients involved with trunk and femoralgenital region, the positive rate was  78.6% (77/98). Whereas in patients involved with  hands and feet, positive rate was  75.0%  (39/52). As regards to the fingers/toes region, positive rate was 59.5% (25/42). With  KOH wet positive method:the positive rate was 53.6% (103/192). The positive rate in the trunk region,in hands and feet and in fingers/toes was 60.2%(59/98),53.8%(28/52),38.1% (16/42), respectively. The positive rate of fluorescent fungus staining test was  significantly  higher than those of KOH wet method (X²=16.23,P<0.05). Relative to  KOH wet method,the specificity of fluorescent staining method to detect regions of trunk, feet and fingers/toes region  were 53.8%, 54.2% and 65.4%,respectively, and the sensitivity was 100%. Conclusion:As for inexperienced technician, fluorescent staining is easy to perform, with a high accuracy. The fungi figures are  easily  distinguishable even to patients, resulting in high patient compliance. Therefore, it is applicable to clinic work.