Objective To elucidate the anti-inflammatory effects and underlying molecular mechanisms of recombinant ANKRD22 protein in dendritic cells, and to assess its therapeutic efficacy in mitigating the progression of psoriasis. Methods In vitro, mouse bone marrow cells isolated from femurs and tibias were induced into mature bone marrow-derived dendritic cells (BMDC) using GM-CSF. The BMDC were divided into four groups: PBS, IMQ, PBS+recombinant ANKRD22, and IMQ+recombinant ANKRD22. Each group was treated accordingly with PBS, IMQ, or recombinant ANKRD22 protein for 24 h. Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression levels of mRNA for IL23a, IL1b, and ANKRD22, while Western blot was performed to detect the protein levels of key members of the noncanonical NF-κB signaling pathway. In vivo, an IMQ-induced mouse model of psoriasis was established (n=10) and divided into a PBS control group (n=5) and a recombinant ANKRD22 group (n=5). The changes in body weight and PASI scores were recorded daily. Histological changes and acanthosis area were evaluated by hematoxylin and eosin (H&E) staining. Flow cytometry was used to determine the proportions of neutrophils, Th17, and γδT17 cells, and qRT-PCR was used to quantify the expression levels of mRNA for IL1b,IL23a,IL17a,IL17f,IL6 and TNF-α in the skin. Results In vitro, compared with the PBS group, expression levels of IL23a, IL1b and ANKRD22 mRNA in the IMQ group significantly increased to 1.97±0.33(P=0.007), 1.80±0.32(P=0.014)and 2.34±0.52(P=0.014)fold, accompanied by enhanced NIK accumulation and downstream p52/RelB nuclear translocation. In contrast, compared with the IMQ group, IL23a expression in the IMQ+recombinant ANKRD22 group was significantly reduced, from 1.97±0.33 to 1.17±0.19-fold (P=0.023), without significant differences in the expression levels of IL1b and ANKRD22 mRNA. Concurrently, NIK accumulation and downstream p52/RelB nuclear translocation were markedly attenuated. In vivo, recombinant ANKRD22 significantly alleviated psoriasis-like symptoms. On day 5, the PASI score was significantly lower in the recombinant ANKRD22 group than in the PBS group (6.10±0.65 vs. 7.80±0.83, P=0.007), acanthosis area was reduced from 0.08±0.01 to 0.04±0.01 mm² (P=0.001), and splenomegaly was mitigated with an increased body weight (20.24±0.51 g vs. 19.06±0.45 g, P=0.005). The proportions of neutrophils, Th17, and γδT17 cells in the skin decreased from (21.00±3.07)%, (38.16±3.66)%, and (9.74±3.02)% to (6.48±1.96)%(P=0.001), (24.48±3.37)%(P=0.001), (6.42±1.55)%(P=0.030), respectively. The expression levels of mRNA for IL1b, IL23a, IL17a, IL17f, IL6 and TNF-α in the skin were also reduced from (32.67±17.34)% to (67.37±25.43)%. Conclusions Recombinant ANKRD22 protein suppresses IL-23 production by selectively inhibiting the noncanonical NF-κB signaling pathway in dendritic cells, thereby blocking the IL-23-Th17/γδT17 immune axis, promoting inflammatory resolution, and effectively ameliorating psoriasis progression.

Objective To assess the efficacy and safety of apremilast in the treatment of plaque psoriasis. Methods Fifty patients with plaque psoriasis were enrolled and categorized into mild, moderate, and severe groups based on disease severity. They received 12 weeks of treatment with apremilast. The psoriasis area and severity index (PASI) scores, as well as PASI50, PASI75, and PASI90, were recorded at weeks 0, 4, 8, and 12. Additionally, the incidence and severity of adverse events were recorded. Results Fifty patients included 12 mild cases, 33 moderate cases, and 5 severe cases. At weeks 4, 8, and 12, the PASI scores for mild (0.60±0.40, 0.34±0.29, 0.27±0.26), moderate (2.43±1.17, 1.55±0.94, 1.06±0.83), and severe (7.04±0.94, 5.26±1.48, 4.18±1.55) groups significantly decreased from baseline. The PASI50, PASI75, and PASI90 were highest in the mild group (PASI50: 75%, 100%, 100%; PASI75: 42%, 75%, 75%; PASI90: 0, 17%, 50%), while they were lowest in the severe group. Fifty-six percent of patients reported at least one adverse event during the 12-week period, with the most common being diarrhea (30%), nausea (12%), and muscle pain (6%). Few patients experienced depression, headache, stomachache, or toothache. No serious adverse events occurred. Conclusions Apremilast demonstrates good efficacy for mild to moderate plaque psoriasis. Diarrhea is the most commonly reported adverse event and is generally well tolerated.

Objective To evaluate the clinical efficacy and safety of intralesional injection of heparin sodium versus carbon dioxide (CO₂) laser ablation in the treatment of xanthelasma palpebrarum. Methods A total of 38 patients with Grade Ⅰ-Ⅲ xanthelasma and normal blood lipid levels were retrospectively enrolled, and divided into two groups based on the treatment modality: the heparin sodium group (n=20) treated with intralesional injection of heparin sodium and the CO₂ laser group (n=18) treated with CO₂ laser ablation. The primary and secondary outcomes were compared between the two groups, including the therapeutic effect at 1 week post-treatment, the recurrence rate at 3 months, the incidence of adverse events during treatment and follow-up, and patient satisfaction. Results There was no significant difference in the overall therapeutic effect between the two groups (P=0.107). All patients in the CO₂ laser group achieved excellent response (100%), while 80.00% of patients in the heparin sodium group achieved excellent response, and 20.00% achieved good response. Regarding patient satisfaction, the satisfaction rate was higher in the heparin sodium group than in the CO₂ laser group (100% vs. 83.33%, P=0.023). For adverse events, the incidence of scar formation (72.22%) and hypopigmentation (33.33%) in the CO₂ laser group was significantly higher than that in the heparin sodium group (both P<0.01). In contrast, the incidence of hyperpigmentation in the heparin sodium group was higher than that in the CO₂ laser group (60.00% vs. 5.56%, P<0.001), and most cases of hyperpigmentation spontaneously resolved or improved within 6 months. No statistically significant difference was observed in the recurrence rates between the two groups (P=0.663). Conclusions Intralesional injection of heparin sodium for xanthelasma offers comprehensive advantages, including “definite efficacy, no risk of scarring, and high treatment satisfaction,” making it a potentially viable alternative for treating xanthelasma.

Objective To evaluate the awareness of the HPV vaccine among healthcare workers (HCWs) in dermatology (STD), infectious diseases, and related departments, their attitudes toward male vaccination and recommendation willingness, and to identify associated determinants. Methods This survey was conducted in Guangzhou and Shenzhen from October to November 2024. Electronic questionnaires were distributed via "questionnaire star" to HCWs in dermatology (STD), infectious diseases, and related departments. The instrument comprised three modules: (1) sociodemographic characteristics; (2) knowledge, attitudes, and behaviors regarding HPV and HPV vaccination; and (3) the current status of recommending HPV vaccination for males and willingness to recommend it. Results A total of 94 valid questionnaires were collected, primarily from doctors in infectious diseases and dermatology-STD departments at public hospitals (72.34%). Overall, HCWs demonstrated high awareness of HPV and the HPV vaccine, with the vast majority (91.49%) believing that males should be vaccinated. The HPV vaccination rate among female HCWs was 56.25% (36/64), with a higher rate (65.38%, 34/52) in those under 45 years old. No male HCWs had received the HPV vaccine. Most HCWs were willing to recommend the HPV vaccine to their partners (88.30%), children (87.23%), friends (92.55%), and male patients (95.74%). 67.02% of the HCWs had recommended the HPV vaccine to male patients, with high patient acceptance(76.19%). 82.98% of the HCWs had received HPV vaccine-related inquiries, and 92.55% believed their knowledge could benefit patients. Conclusions HCWs among dermatology and infectious diseases have a relatively high awareness of HPV and its vaccines. They also show a strong willingness to recommend the HPV vaccine for males. However, the vaccination rate among themselves still needs improvement. There is a need to enhance health education specifically aimed at increasing awareness of the necessity and importance of HPV vaccination for males, in order to improve the vaccination rate among this group.

Objective To synthesize current evidence on wound management in patients with pemphigus, providing an evidence-based foundation for clinical nursing practice. Methods According to the "6S" pyramid model, a systematic search of domestic and international databases, as well as relevant websites, was conducted for literature published from inception to April 2025 on wound management in patients with pemphigus. Following the assessment of the retrieved studies, the content from eligible studies that met the quality standards based on the themes was extracted and synthesized to form an evidence summary for wound management in patients with pemphigus. Results A total of 15 studies were included, comprising 3 clinical decision aids, 5 guidelines, 2 systematic reviews, 2 expert consensuses, and 3 randomized controlled trials. Thirty-four evidence statements were derived across five domains: wound assessment, wound cleansing techniques, management of bullae and erosion, selection and application of dressings, and nutritional support. Conclusions The evidence-based medical evidence integrated in this study can provide scientific reference for clinical healthcare professionals in developing wound management strategies for patients with pemphigus.

Objective To analyze the epidemic trend and population distribution characteristics of syphilis in the Zhongshan City of Guangdong Province, so as to provide a scientific basis for formulating targeted prevention and control strategies. Methods Based on the data from the National Notifiable Infectious Diseases Reporting System between 2015 and 2024, the reported incidence rates of syphilis were stratified and organized by year, stage, gender, and age group. The Joinpoint regression model was used to analyze the annual percentage change (APC) and trend inflection points. Results From 2015 to 2024, the reported incidence rate of syphilis decreased from 61.0 per 100,000 population to 56.7 per 100,000 population (AAPC=-2.2%, P=0.581), showing a continuous downward trend. Primary and secondary syphilis decreased by 83.8% and 35.1%, respectively, while latent syphilis increased by 22.0%, contrary to the trend (from 41.4 per 100,000 to 50.5 per 100,000), accounting for as much as 89.1%. The incidence rates decreased simultaneously in both genders, and the reported incidence rate among people aged 60 and above was significantly higher than that in other age groups. Conclusions The prevalence of syphilis in Zhongshan City, Guangdong Province, shows an overall controlled and slightly declining trend, while there is a notable increase in the number of asymptomatic carriers and a high incidence among the elderly aged 60 and above. This indicates the need to improve the three-level prevention and control system, strengthen monitoring and early intervention management for asymptomatic carriers, and curb hidden transmission. It is essential to focus on women and youth, promote knowledge about sexually transmitted disease prevention and control, and enhance the risk awareness of key populations. Additionally, it is important to optimize and innovate prevention and control strategies for the elderly, develop accessible popular science materials tailored to older adults, strengthen health education, and increase public risk awareness and protective capabilities accordingly.

We present a case of cutaneous metastatic carcinoma that was initially misdiagnosed as herpes zoster. A 74-year-old male experienced painful erythemas, papules, and nodules on the right thoracic and dorsal regions for over two months. He had a history of small cell lung cancer with multiple lymph node metastases for more than two years. Prior to this visit, he had been receiving anti-tumor treatment with adibelimab and docetaxel. Dermatological examination showed a band-like distribution of erythemas, papules, and nodules on the right chest and back. The lesions appeared pale red to pink, smooth, and firm in texture, with some resembling a "pseudobullous" appearance. Histopathological examination of the skin lesion showed atypical cell infiltration in the subcutaneous fibrous stroma. Immunohistochemistry demonstrated CK(+) and P40(+). Diagnosis: Metastatic cutaneous squamous cell carcinoma (T4N3M1 Stage Ⅳ). The oncology department administered IMRT radiotherapy to the thoracic and dorsal skin areas, at a dose of 60Gy/30f. A comprehensive assessment of the patient's multiple pulmonary metastases indicated end-stage cancer. Despite aggressive treatment, the disease was poorly controlled, leading to death.

We report a case of kerion celsi caused by Microsporum canis in a child. A 2-year-old girl presented with multiple pustules, edematous plaques, scalp alopecia, and pustular scabs for more than 1 month. The fungal microscopic examination was positive. Fungal culture of the broken hair showed positive results for Microsporum canis. Molecular biology identified the fungus Microsporum canis, and the Brucella tube condensation test was positive ++(1 ∶400).Diagnosis:①Kerion Celsi caused by Microsporum canis; ②Brucellosis. After 8-week treatments with oral Griseofulvin combined with Compound Glycyrrhizin tablets, supplemented with ozone water rinses and standardized topical antifungal medication, the tinea capitis resolved, and follow-up tests showed negative fungal cultures. During six-month follow-up, there were no recurrences, and hair regrowth was satisfactory. The patient is currently continuing oral Mongolian medicine treatment for brucellosis.

Acne is a common chronic, inflammatory disease of the pilosebaceous glands. Its pathogenesis is complex and affected by many factors. In recent years, the research on the pathogenesis of acne has been deepened. As a pleiotropic growth factor, insulin-like growth factor-1 (IGF-1) plays an important role in the pathogenesis of acne. Studies have shown that IGF-1 can promote the proliferation and lipid synthesis of sebaceous gland cells by activating PI3K/Akt/mTORC1 signaling pathway, while also increasing androgen receptor sensitivity and aggravating sebaceous hypersecretion. This article reviews the current research progress on the role of IGF-1-mediated inflammation in the pathogenesis of acne.

Vitiligo is a common depigmenting skin disorder with a complex pathogenesis, primarily resulting from the destruction, death, or dysfunction of melanocytes. In recent years, ferroptosis, a novel iron-dependent form of cell death driven by lipid peroxidation, has been recognized as playing a significant role in the onset and progression of vitiligo. Factors such as iron homeostasis imbalance, lipid peroxidation accumulation, weakened antioxidant defenses, and activation of immune inflammation collectively contribute to melanocyte damage. This review systematically examines the physiological processes of iron metabolism, explores the molecular mechanisms of ferroptosis in melanocyte injury and its role in vitiligo pathogenesis, and summarizes potential therapeutic targets and drug interventions related to ferroptosis. An in-depth exploration of the interactions between ferroptosis and immune regulation provides novel insights and a strong theoretical foundation for the precise prevention and treatment of vitiligo.

Specialized pro-resolving mediators (SPMs) are endogenous lipid molecules that are biosynthesized from ω-3/ω-6 polyunsaturated fatty acids via key enzymes such as lipoxygenases and cyclooxygenases. By targeting specific receptors on the surfaces of immune cells, SPMs precisely regulate inflammation resolution and play a crucial role in maintaining skin homeostasis. This review delineates the dynamic changes and regulatory roles of SPMs in various physiological and pathological processes, including psoriasis, atopic dermatitis, skin wound healing, and skin aging. Specifically, certain SPMs, such as RvD5 and PDx, are detectable in psoriatic lesions, while an imbalance between pro-inflammatory mediators and SPMs is observed in the skin of patients with atopic dermatitis. During skin wound healing, the content of the resolvin D series increases, promoting inflammation resolution and tissue repair. In the process of skin aging, the downstream metabolism of EPA/DHA in aged skin fails to effectively produce active SPMs, leading to a decline in the skin's ability to resolve inflammation. Furthermore, this article elucidates the role of SPMs in alleviating skin inflammatory symptoms such as pain, pruritus, edema, and erythema through mechanisms that include regulating transient receptor potential (TRP) channels, inhibiting the release of pro-inflammatory factors, and modulating vascular permeability. It provides a theoretical basis for the clinical application of novel skin anti-inflammatory strategies based on SPMs or their analogs.