Objective To investigate the localization of Tp0844 on Treponema pallidum and its role in adhesion. Methods The protein sequence of Tp0844 from the T. pallidum Nichols strain was retrieved from GenBank. Molecular weight, isoelectric point, signal peptide, and phosphorylation sites were predicted using DTU Health Tech tools. Multiple sequence alignment was performed using MEGA11 and visualized using Jalview, while the three-dimensional structure was predicted by AlphaFold3. The pET28a(+)-tp0844 vector was constructed, and the recombinant rTp0844 protein was expressed in vitro and confirmed by western blotting. Mice were immunized with rTp0844 to generate polyclonal antibodies. Indirect immunofluorescence staining was used to assess the localization of Tp0844 on T. pallidum,with without Tp antibody treatment as the control group and Tp-Ab as the positive control group. The antibody's adhesion-blocking effect was evaluated by dark-field microscopy enumeration and quantitative PCR after incubating T. pallidum with Sf1Ep cells, with the PBS-treated group as the control group. Results Tp0844 consists of 350 amino acids, with a predicted molecular weight of approximately 38.09 kD and an isoelectric point of 8.16. Amino acid sequence identities with GAPDH from humans, Mycobacterium tuberculosis, Streptococcus pneumoniae, Mycoplasma pneumoniae, and Neisseria meningitidis were 47.58%, 48.29%, 42.74%, 44.13%, and 44.19%, respectively. SignalP 5.0 predicted no signal peptide cleavage site, while NetPhosBac-1.0 identified nine serine/threonine phosphorylation sites. Multiple sequence alignment revealed conservation of C-terminal residues among GAPDH orthologs from different pathogens. AlphaFold3 prediction indicated the absence of a typical β-barrel structure characteristic of T. pallidum outer membrane proteins. Western blot confirmed the successful construction and expression of the recombinant Tp0844 protein. Indirect immunofluorescence showed no fluorescence in the blank control group, whereas the Tp0844 group exhibited green fluorescence similar in size and morphology to the positive control (Tp-Ab group). Compared with the control group, the rTp0844 antibody-treated group exhibited no significant change in the number of treponemes in the cell supernatant (P>0.05), and the copy number of Treponema pallidum DNA in the cell culture supernatant increased (t=3.10, P=0.018), indicating reduced binding to Sf1Ep cells. Conclusion Tp0844 is localized on the outer membrane of Treponema pallidum and mediates its adhesion to host cells.

Objective To elucidate the targets and mechanisms of action of Taohong Fuse Formula (THF) in the treatment of vitiligo using integrated network pharmacology and in vivo animal experiments. Methods Active constituents and targets of the 11-herb THF were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). SwissTargetPrediction was used to screen THF active constituents and predict potential targets, while vitiligo-related targets were obtained from GeneCards, Online Mendelian Inheritance in Man (OMIM), and the Therapeutic Target Database (TTD). A protein-protein interaction (PPI) network was constructed to identify core targets, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Subsequently, a “herb-component-target” network was established. In vivo, vitiligo was induced in mice using 2.5% hydroquinone cream for 4 weeks. Sixty C57BL/6 mice were randomly divided into six groups: the blank control group, the model group, the low-, medium-, and high-dose traditional Chinese medicine groups (with drug concentrations of 0.76 mg/mL, 1.55 mg/mL, and 3.10 mg/mL, respectively), and the Western medicine group (prednisone acetate at 0.27 mg/mL). Each group 10 mice. Except for the blank control group, 5 mg of 2.5% hydroquinone cream was evenly applied to the back of the mice in the other groups once daily for 4 weeks. Then, four groups (n=10) were treated with low-, medium-, or high-dose THF, or prednisone for 40 days. Dorsal fur changes were monitored, skin pathology was assessed by HE staining, and IL-6, NF-κB p65, and p-NF-κB p65 protein levels in skin tissue were measured by Western blot. Results THF exerts therapeutic effects on vitiligo primarily through key active components such as β-sitosterol, luteolin, kaempferol, and quercetin, targeting IL-6, TNF, and AKT1. GO and KEGG enrichment analyses indicated that these targets regulate immune and inflammatory responses via the PI3K-Akt, TNF, and NF-κB signaling pathways. Compared with the model group, THF intervention ameliorated skin pathological injury, increased melanocyte numbers, and significantly downregulated IL-6 and p-NF-κB p65 protein expression. Medium dose may be the eptimal effectire dose. Conclusion THF acts on vitiligo through multiple components and targets, primarily by inhibiting the NF-κB signaling pathway.

Objective To investigate the association between syphilis infection and the distribution characteristics of different types of malignant tumors. Methods A retrospective Cross-sectional study was conducted using data from 113 520 inpatients who underwent syphilis serological testing at Zhongshan Hospital Affiliated to Xiamen University from January 2015 to December 2025. Patients were divided into a syphilis-positive group (n=3 487) and a syphilis-negative group (n=110 033). We compared differences in the proportions of malignant tumors and 14 common tumor subtypes between the two groups. Genderrelated differences and agedistribution trends of tumors among syphilispositive patients were analyzed. Results The proportion of malignant tumors was significantly higher in the syphilis-positive group than in the syphilis-negative group (19.10% vs. 13.47%), with an OR of 1.52 (95%CI: 1.39-1.66, $\chi $²=91.1, P<0.05). The syphilis-positive group showed markedly increased risks of prostate cancer (OR=2.71, 95%CI: 1.62-4.53), cervical cancer (OR=1.61, 95%CI: 1.22-2.12), and lung cancer (OR=1.58, 95%CI: 1.17-2.14), whereas the proportion of thyroid cancer was significantly decreased (OR=0.32, 95%CI: 0.14-0.71). Genderstratified analysis revealed that among syphilis-positive patients, the proportions of bladder cancer, gastrointestinal cancer, and liver cancer were significantly higher in males than in females (P<0.05). Age trend analysis showed that the proportion of patients aged ≥61 years with lung cancer and thyroid cancer exhibited a significant increasing trend in the syphilis-positive group. Conclusions In this single-center inpatient population, syphilis-positive patients had higher proportions of multiple malignant tumors, including prostate cancer, cervical cancer, and lung cancer. Malignant tumor distribution differed by gender among syphilis-positive patients, and increased proportions of certain tumors were observed in the elderly group. Enhanced screening for relevant malignancies is recommended for syphilis-positive patients, particularly middle-aged and elderly males.

Objective To evaluate the effect of functional lip care products on the skin physiological condition of individuals with lip sensitivity, providing a scientific basis for precise care of sensitive lip skin. Methods Thirty-five subjects with a history of lip hypersensitivity were enrolled and instructed to apply the functional lip care product for 14 consecutive days. Core parameters, including lip wrinkles, stratum corneum hydration, transepidermal water loss (TEWL), melanin index, erythema index, lip gloss and lip elasticity, were measured using non-invasive cutaneous measurement devices before and after product use. In conjunction with visual assessment by dermatologists, the product's overall efficacy was systematically evaluated. Results Five minutes after initial use, as well as on days 1, 7 and 14 of use, significant reductions in the length, area and volume of lip wrinkles were observed (P<0.05). On days 1, 7 and 14 of use, TEWL decreased continuously (a reduction of 36.10%), melanin index declined continuously (a reduction of 32.39%) (P<0.01), glossiness and skin elasticity parameters (R2, R7, Q1) increased significantly (P<0.05); On days 1 and 14 of use, stratum corneum hydration increased significantly (an increase of 11.67%) (P<0.01); Erythema index decreased significantly on Days 1 and 7 of use (P<0.01). Physician visual assessment showed significantly decreased scores for lip dryness, desquamation, pruritus, and dullness, while scores for glossiness, elasticity, and firmness were significantly increased (P<0.01). Conclusions Functional lip care products can quickly reduce lip lines, effectively repair the lip skin barrier, alleviate sensitivity-related symptoms, improve lip color dullness, and enhance lip elasticity and gloss, making them suitable for daily care for individuals with sensitive lips.

Objective To investigate the value of a precision perioperative nursing model in patients with dermatofibrosarcoma protuberans (DFSP) undergoing Mohs micrographic surgery. Methods We conducted a retrospective analysis of clinical data from 250 patients with DFSP at our hospital from February to December 2025. All patients underwent Mohs micrographic surgery combined with flap/skin graft reconstruction. We implemented a precision perioperative nursing system, including preoperative multidimensional risk screening and cognitive intervention; intraoperative refined nursing cooperation; postoperative three-dimensional dynamic skin tension management based on the "site-stage-activity" framework; modified negative-pressure drainage; and integrated Chinese and Western medicine closed-loop pain management. We analyzed hospitalization duration, nursing satisfaction, pain control efficacy, and complications. Results 250 patients successfully completed the surgery, with all incisions achieving primary healing. The hospitalization duration was 7.0±1.6 days, and the nursing satisfaction rate was 100%. After postoperative pain pre-intervention, 211 cases (84.4%) reported no pain on the numerical rating scale (NRS). Among the patients requiring additional pain intervention, all achieved satisfactory pain control, with a pain control satisfaction rate of 100%. Venous crisis occurred in 8 cases (3.2%), all of which were completely resolved after timely management. No severe complications such as flap necrosis or surgical site infection were observed. Conclusion The precision perioperative nursing model can effectively control postoperative pain, reduce flap-related complications, and promote wound healing in DFSP patients undergoing Mohs micrographic surgery, warranting broader clinical application.

A case of angiolymphoid hyperplasia with eosinophilia is presented. A 55-year-old female exhibited pruritic papules and nodules on the left scalp, postauricular area, and upper limbs for six months. Dermatological examination identified multiple red or dark red hemispherical papules and nodules of varying sizes on the left occipital region, postauricular area, and extensor surfaces of the upper limbs. The largest lesion measured approximately 0.6 cm in diameter, with a firm consistency, well-defined borders, and a crusted surface. No tenderness was detected upon palpation. Histopathological examination revealed an exophytic nodule with epidermal ulceration. The dermis exhibited extensive vascular proliferation and dilatation, with swollen epithelioid endothelial cells protruding into the vascular lumen. Abundant infiltration of lymphocytes and eosinophils was observed around the vessels. Numerous thick-walled muscular vessels were present in the deep dermis.Immunohistochemically, staining was positive for CD31, ERG, FLI1, SMA and CD3; CD20 and EMA showed scattered positivity, and PCK was negative.The diagnosis of angiolymphoid hyperplasia with eosinophilia was confirmed. Treatment included surgical excision and intramuscular injection of triamcinolone acetonide. No recurrence was observed during a one-year follow-up.

We report a case of right facial Merkel-cell carcinoma. A 57-year-old female patient presented with a 3-month history of cutaneous nodules on her right cheek. Dermatological examination revealed an oval, dark purplish-red nodule approximately 3 cm×2 cm in size on the right cheek. The lesion was elevated above the skin surface, hard-textured, well-demarcated, poorly mobile, tender to palpation, and smooth-surfaced. Several soybean-sized enlarged lymph nodes were palpable in the right cervical region, while no other superficial lymphadenopathy was detected. Histopathological examination of the skin lesion showed tumor cells in the dermis, arranged in nests or sheets. The tumor cells had scant cytoplasm, oval nuclei, fine chromatin, and exhibited invasive growth. Immunohistochemical staining demonstrated characteristic perinuclear dot-like positivity for CK and CK20, CD56 (+++), Syn (++), INSM1 and SSTR2 were positive, with a markedly elevated Ki-67 proliferation index (>95%+). Immunohistochemistry identified no metastatic tumor cells in cervical lymph nodes. Final diagnosis: Merkel-cell carcinoma of the right face. The patient received 3 cycles of chemotherapy with carboplatin, etoposide (VP-16), and adbelib, followed by extended resection of the facial tumor and right cervical lymph node dissection. The patient recovered uneventfully after surgery, and no recurrence was noted during the 2-month follow-up.

Resveratrol is a natural polyphenolic compound with multiple biological activities, which has shown great therapeutic potential in skin diseases. As understanding of skin disease pathogenesis deepens, resveratrol has attracted much attention because of its excellent antioxidant, anti-inflammatory, anti-aging, and anti-proliferative properties. Current research has revealed the molecular basis for resveratrol's therapeutic effect by regulating core signaling pathways such as SIRT1, NF-κB, and Nrf2. Still, low bioavailability is the main bottleneck limiting its clinical translation. This review aims to systematically elucidate the mechanism of action and clinical application progress of resveratrol in various skin diseases such as acne, psoriasis, atopic dermatitis, photoaging, cancer, and wound healing, and explore the latest research on novel delivery technologies, such as nanocarriers and liposomes, to overcome these application barriers and aims to provide a comprehensive theoretical basis and future development directions for resveratrol from basic research to clinical practice.

Solar urticaria (SU) is a rare form of chronic inducible urticaria triggered by exposure to specific wavelengths of ultraviolet or visible light. It carries a high disease burden, and complete symptom control with conventional antihistamines remains limited. Omalizumab is a humanized monoclonal antibody targeting IgE. By binding free IgE in a non-antigen-specific manner, it reduces IgE availability and downregulates FcεRI expression on mast cells and basophils, thereby inhibiting the IgE/FcεRI-mediated allergic cascade involved in SU. Recent case series, cohort studies, real-world data and systematic reviews suggest that omalizumab 300 mg every 4 weeks can achieve complete or good response in many patients with refractory SU, improve photo provocation test outcomes and quality of life, and is generally well tolerated. However, current evidence is mainly derived from case reports, small cohorts and retrospective studies; long-term safety, optimal tapering strategies and cost-effectiveness remain to be clarified. This review summarizes the mechanism, dosing regimens, clinical efficacy, safety and evidence gaps of omalizumab in SU.

Vaccination is an effective method for preventing infectious diseases in public health, and cutaneous reactions represent the most common adverse effect. Cutaneous adverse reactions are classified as local or systemic based on anatomical location, and as type Ⅰ, type Ⅲ, type Ⅳ hypersensitivity reactions and non-immune reactions based on immune mechanisms; they are also classified as mild, moderate, severe, or extremely severe based on severity. Specifically, mild-to-moderate reactions are managed with symptomatic care; severe cutaneous adverse reactions require immediate withdrawal of the suspected trigger, early referral, multidisciplinary supportive care, and personalized immunomodulatory therapy, including Stevens-Johnson syndrome and toxic epidermal necrolysis. Here, we systematically reviewed the classification, pathogenesis, clinical manifestations, and treatment strategies of cutaneous adverse reactions following vaccination, providing a reference for clinicians and vaccination care.